Vibratory stimulation enhances thyroid epithelial cell function.

The tissues of the body are routinely subjected to various forms of mechanical vibration, the frequency, amplitude, and duration of which can contribute both positively and negatively to human health. The vocal cords, which are in close proximity to the thyroid, may also supply the thyroid with important mechanical signals that modulate hormone production via mechanical vibrations from phonation. In order to explore the possibility that vibrational stimulation from vocalization can enhance thyroid epithelial cell function, FRTL-5 rat thyroid cells were subjected to either chemical stimulation with thyroid stimulating hormone (TSH), mechanical stimulation with physiological vibrations, or a combination of the two, all in a well-characterized, torsional rheometer-bioreactor. The FRTL-5 cells responded to mechanical stimulation with significantly (p<0.05) increased metabolic activity, significantly (p<0.05) increased ROS production, and increased gene expression of thyroglobulin and sodium-iodide symporter compared to un-stimulated controls, and showed an equivalent or greater response than TSH only stimulated cells. Furthermore, the combination of TSH and oscillatory motion produced a greater response than mechanical or chemical stimulation alone. Taken together, these results suggest that mechanical vibrations could provide stimulatory cues that help maintain thyroid function.

Strain distribution in an elastic substrate vibrated in a bioreactor for vocal fold tissue engineering.

A bioreactor previously described was used to quantify the shear strain along a bioengineered tissue scaffold driven at low audio frequencies (20-200 Hz). Standing wave patterns were calculated analytically by solving a classical boundary value problem for a vibrating string under tension and bending stiffness. Boundary conditions were non-traditional in that small pivot arms at the endpoints allowed neither the displacement nor the velocity to go to zero. The calculations were corroborated with stroboscopic measurement of the motion of the material in the bioreactor. Results indicate that shear strains up to 0.2 can be obtained at low frequencies (20 Hz), with a gradual decrease at higher frequencies due to the decaying amplitude response of the mechanical driver. The bioreactor may be useful for approximating the Young's modulus of the material in situ by probing for resonance frequencies in the standing wave pattern. A yet unsolved problem is a variable drag coefficient along the length of the material due to fluid turbulence in the culture medium.

Vocal intensity in falsetto phonation of a countertenor: an analysis by synthesis approach.

An analysis by synthesis paradigm was implemented to model glottal airflow and vocal tract acoustics for the falsetto phonation of a trained countertenor. Changes in vocal intensity were measured as a function of subglottal pressure, open quotient of the time-varying glottal airflow pulse, and formant tuning. The contributions of laryngeal adduction (open quotient of the glottal flow pulse) and of formant tuning to intensity change were derived from modeled data. The findings were: (1) Subglottal pressure accounted for almost 90% of the variation in SPL in falsetto phonation. (2) The open quotient of the glottal flow pulse was remarkably constant in these falsetto phonations, and thus did not affect vocal intensity significantly. (3) Formant tuning occurred in two out of nine possibilities for the vowel /a/. These instances did not support the concept of systematic exploitation of formant tuning.

Perception of pitch and roughness in vocal signals with subharmonics.

Pitch and roughness were rated according to the extent of amplitude modulation (AM) and frequency modulation (FM) of a subharmonic [fundamental frequency (F0)/2]. The objective was to determine the identification boundaries for pitch and roughness and to discover how both kinds of modulation affect these boundaries. Another objective was to judge the reliability between subjects when identifying subharmonic-related pitch and roughness. Three procedures were used: ABX comparisons, method of adjustment, and rating of roughness. Results indicated that the crossover point to the lower pitch (associated with the subharmonic) occurred between 10% and 30% modulation, depending on modulation type and F0. Subjects demonstrated highly variable perceptions of pitch and roughness, with poor intersubject reliability.

The effect of cricothyroid muscle action on the relation between subglottal pressure and fundamental frequency in an in vivo canine model.

The relation between subglottal pressure (Ps) and fundamental frequency (F0) in phonation was investigated with an in vivo canine model. Direct muscle stimulation was used in addition to brain stimulation. This allowed the Ps-F0 slope to be quantified in terms of cricothyroid muscle activity. Results showed that, for ranges of 0-2 mA constant current stimulation of the cricothyroid muscle, the Ps-F0 slope ranged from 10 Hz/kPa to 60 Hz/kPa. These results were compared to similar slopes obtained in a previous study on excised larynges in which the vocal fold length was varied instead of cricothyroid activation. A physical interpretation of the Ps-F0 slope is that the amplitude-to-length ratio of the vocal folds decreases with CT activity, resulting in a smaller time-varying stiffness. In other words, there is less dependence of F0 on amplitude of vibration when the vocal folds are long instead of short.

Preparation of the speech-language pathologist specializing in voice: an educational survey.

This study investigates the academic and clinical preparation a speech-language pathologist receives in the area of voice. The purpose is to determine how to structure continuing education and specialty training in vocology, defined as the science and practice of voice habilitation. Surveys were sent to the graduate programs in speech-language pathology accredited by the American Speech-Language-Hearing Association (ASHA) in 1994, and again in 1999. Results from the 1994 survey indicated that students received limited information and clinical experience in handling typical voice cases. Although most programs required a voice disorders course, fewer mandated coursework in normal voice production or clinical experience with voice patients. The follow-up survey showed that the educational environment in 1999 is similar to that in 1994. It is evident that the programs are in compliance with the Educational Training Board model developed by ASHA for basic clinical competence, but no structure for specialty training has yet emerged.

The importance of hyaluronic acid in vocal fold biomechanics.

OBJECTIVE: This study examined the influence of hyaluronic acid (HA) on the biomechanical properties of the human vocal fold cover (the superficial layer of the lamina propria). STUDY DESIGN: Vocal fold tissues were freshly excised from 5 adult male cadavers and were treated with bovine testicular hyaluronidase to selectively remove HA from the lamina propria extracellular matrix (ECM). Linear viscoelastic shear properties (elastic shear modulus and dynamic viscosity) of the tissue samples before and after enzymatic treatment were quantified as a function of frequency (0.01 to 15 Hz) by a parallel-plate rotational rheometer at 37 degrees C. RESULTS: On removing HA from the vocal fold ECM, the elastic shear modulus (G' ) or stiffness of the vocal fold cover decreased by an average of around 35%, while the dynamic viscosity (eta') increased by 70% at higher frequencies (>1 Hz). CONCLUSION: The results suggested that HA plays an important role in determining the biomechanical properties of the vocal fold cover. As a highly hydrated glycosaminoglycan in the vocal fold ECM, it likely contributes to the maintenance of an optimal tissue viscosity that may facilitate phonation, and an optimal tissue stiffness that may be important for vocal fundamental frequency control. SIGNIFICANCE: HA has been proposed as a potential bioimplant for the surgical repair of vocal fold ECM defects (eg, vocal fold scarring and sulcus vocalis). Our results suggested that such clinical use may be potentially optimal for voice production from a biomechanical perspective.

Characterization of the medial surface of the vocal folds.

A method is developed for the quantification of the medial surface of the vocal folds in excised larynges. Lead molds were constructed from the glottal airway of a canine larynx for 3 distinct glottal configurations corresponding to "pressed" folds, just barely adducted folds, and 1-mm-abducted folds as measured between the vocal processes. With a high-resolution laser striping system, the 3-dimensional molds were digitally scanned. Low-order polynomials were fitted to the data, and goodness-of-fit statistics were reported. For all glottal configurations, a linear variation (flat surface) approximated the data with a coefficient of determination of 90%. This coefficient increased to roughly 95% when a quadratic variation (curvature) was included along the vertical dimension. If more than the top 5 mm or so of the folds was included (the portion usually corresponding to vibration), a cubic variation along the vertical dimension was necessary to explain a change in concavity at the conus elasticus. These findings suggest the utility of a model based on a convergence coefficient and a bulging coefficient. For all glottal configurations, the convergence coefficients and bulging coefficients can be computed. Because pre-phonatory conditions have a profound influence on vocal fold vibration and on the quality of phonation, such shaping parameters are highly significant. With the viability of this method substantiated, it is envisioned that future studies will characterize greater quantities of glottal shapes, including those of human vocal folds.

The relationship of vocal tract shape to three voice qualities.

Three-dimensional vocal tract shapes and consequent area functions representing the vowels [i, ae, a, u] have been obtained from one male and one female speaker using magnetic resonance imaging (MRI). The two speakers were trained vocal performers and both were adept at manipulation of vocal tract shape to alter voice quality. Each vowel was performed three times, each with one of the three voice qualities: normal, yawny, and twangy. The purpose of the study was to determine some ways in which the vocal tract shape can be manipulated to alter voice quality while retaining a desired phonetic quality. To summarize any overall tract shaping tendencies mean area functions were subsequently computed across the four vowels produced within each specific voice quality. Relative to normal speech, both the vowel area functions and mean area functions showed, in general, that the oral cavity is widened and tract length increased for the yawny productions. The twangy vowels were characterized by shortened tract length, widened lip opening, and a slightly constricted oral cavity. The resulting acoustic characteristics of these articulatory alterations consisted of the first two formants (F1 and F2) being close together for all yawny vowels and far apart for all the twangy vowels.

Three-dimensional vocal tract imaging and formant structure: varying vocal register, pitch, and loudness.

Although advances in techniques for image acquisition and analysis have facilitated the direct measurement of three-dimensional vocal tract air space shapes associated with specific speech phonemes, little information is available with regard to changes in three-dimensional (3-D) vocal tract shape as a function of vocal register, pitch, and loudness. In this study, 3-D images of the vocal tract during falsetto and chest register phonations at various pitch and loudness conditions were obtained using electron beam computed tomography (EBCT). Detailed measurements and differences in vocal tract configuration and formant characteristics derived from the eight measured vocal tract shapes are reported.

A quantitative output-cost ratio in voice production.

A quantitative output-cost ratio (OCR) is proposed for objective use in voice production and is defined as the ratio of the acoustic output intensity to the collision intensity of the vocal folds. Measurement of the OCR is demonstrated in a laboratory experiment using 5 excised larynges and a transducer designed for use on human subjects. Data were gathered at constant fundamental frequency (150 Hz). Subglottal pressure was varied from 1.0 to 1.6 kPa, and glottal width at the vocal processes was varied from a pressed condition to a 2-mm gap. The OCR was plotted as a function of glottal width. With no vocal tract, the excised larynx experiments yielded a broad maxima in the OCR curves, across all subglottal pressure conditions, at about 0.6 mm. Computer simulations indicate that sharper maxima may occur when the influence of the vocal tract is taken into account. The potential clinical utility of the OCR is discussed for treatment of a wide range of voice disorders, including those involving both hyper- and hypoadduction.

An investigation of a modal-falsetto register transition hypothesis using helox gas.

This study concerned the effect of the first subglottal formant (F1') on the modal-falsetto register transition in males and females. Phonations using air and a helium-oxygen mixture (helox) were used in a comparative study to tease apart possible acoustic and myoelastic contributions to involuntary register transitions. Recordings of the first subglottal formant and its accompanying bandwidths, and the lower and upper shift point marking the outer boundaries of abrupt register transitions, were obtained via a neck-mounted accelerometer, and analyzed using spectrograms and power spectra on a K-5500 Sona-Graph. The four subjects had their hearing masked bilaterally with speech level noise to increase the likelihood of involuntary register transition via minimized auditory feedback. In three of the four test subjects registration was surmised to be primarily a laryngeal event, as evidenced by the similar frequency dependency of voice breaks in both air and helox. It may be hypothesized that subglottal resonance influenced register transition in the fourth subject, as voice breaks rose with helox-induced phonation; however, this result did not reach statistical significance. Therefore, in this experiment subglottal resonance was not found to have a significant influence on register transition as originally hypothesized.

Acoustic interpretation of resonant voice.

Resonant voice, often described in terms of vibratory sensations in the face, is investigated acoustically by calculating vocal tract inertance. It appears that the ease of production and vibrancy of resonant voice depends more on lowering phonation threshold pressure than on tissue or air resonance in or around the face. Phonation threshold pressure is lowered by increasing air column inertance in the laryngeal vestibule. The fact that the sensations are felt in the face is an indication of effective conversion of aerodynamic energy to acoustic energy, rather than sound resonation in the sinuses or the nasal airways.

Geometric characterization of the laryngeal cartilage framework for the purpose of biomechanical modeling.

Some new anatomic data on the laryngeal cartilage framework have been obtained for the biomechanical modeling of the larynx. This study attempted to define and measure some biomechanically important morphometric features of the laryngeal framework, including both the human and the canine laryngeal frameworks, because the canine larynx has been frequently used as an animal model in gross morphology and in physiological experiments. The larynges of 9 men, 7 women, and 9 dogs were harvested and dissected after death. Linear and angular geometric measurements on the thyroid cartilage, the cricoid cartilage, and the arytenoid cartilage were made with a digital caliper and a protractor, respectively. The results are useful for constructing quantitative biomechanical models of vocal fold vibration and posturing (abduction and adduction), eg, continuum mechanical models and finite-element models of the vocal folds.

Acoustic impedance of an artificially lengthened and constricted vocal tract.

Voice training techniques often make use of exercises involving partial occlusion of the vocal tract, typically at the anterior part of the oral cavity or at the lips. In this study two techniques are investigated: a bilabial fricative and a small diameter hard-walled tube placed between the lips. Because the input acoustic impedance of the vocal tract is known to affect both the shaping of the glottal flow pulse and the vibrational pattern of the vocal folds, a study of the input impedance is an essential step in understanding the benefits of these two techniques. The input acoustic impedance of the vocal tract was investigated theoretically for cases of a vowel, bilabial occlusion (fully closed lips), a bilabial fricative, and artificially lengthening the tract with small diameter tubes. The results indicate that the tubes increase the input impedance in the range of the fundamental frequency of phonation by lowering the first formant frequency to nearly that of the bilabial occlusion (the lower bound on the first formant) while still allowing a continuous airflow. The bilabial fricative also has the effect of lowering the first formant frequency and increasing the low-frequency impedance, but not as effectively as the extension tubes.

Three-dimensional anatomic characterization of the canine laryngeal abductor and adductor musculature.

The biomechanics of vocal fold abduction and adduction during phonation, respiration, and airway protection are not completely understood. Specifically, the rotational and translational forces on the arytenoid cartilages that result from intrinsic laryngeal muscle contraction have not been fully described. Anatomic data on the lines of action and moment arms for the intrinsic laryngeal muscles are also lacking. This study was conducted to quantify the 3-dimensional orientations and the relative cross-sectional areas of the intrinsic abductor and adductor musculature of the canine larynx. Eight canine larynges were used to evaluate the 3 muscles primarily responsible for vocal fold abduction and adduction: the posterior cricoarytenoid, the lateral cricoarytenoid, and the interarytenoid muscles. Each muscle was exposed and divided into discrete fiber bundles whose coordinate positions were digitized in 3-dimensional space. The mass, length, relative cross-sectional area, and angle of orientation for each muscle bundle were obtained to allow for the calculations of average lines of action and moment arms for each muscle. This mapping of the canine laryngeal abductor and adductor musculature provides important anatomic data for use in laryngeal biomechanical modeling. These data may also be useful in surgical procedures such as arytenoid adduction.

Biomechanical and histologic observations of vocal fold fibrous proteins.

This article discusses the molecular composition of the vocal fold and the relationship of fibrous molecules to the biomechanical and physiological performance of the tissue. The components of the extracellular matrix may be divided into fibrous proteins and interstitial proteins. The fibrous proteins, consisting of collagens and elastins, are the focus of this report. Elastin concentration varies by tissue depth in the vocal folds. Variation of elastin by age is reported, but some controversy exists. The biomechanical terms of stress and strain (and stress-strain curves of human vocal folds) are related to the fibrous proteins of the vocal folds. The fibrous proteins, their role in stress, and their effect on the dynamic range of vocal pitch are presented.

Viscoelastic shear properties of human vocal fold mucosa: theoretical characterization based on constitutive modeling.

The viscoelastic shear properties of human vocal fold mucosa (cover) were previously measured as a function of frequency [Chan and Titze, J. Acoust. Soc. Am. 106, 2008-2021 (1999)], but data were obtained only in a frequency range of 0.01-15 Hz, an order of magnitude below typical frequencies of vocal fold oscillation (on the order of 100 Hz). This study represents an attempt to extrapolate the data to higher frequencies based on two viscoelastic theories, (1) a quasilinear viscoelastic theory widely used for the constitutive modeling of the viscoelastic properties of biological tissues [Fung, Biomechanics (Springer-Verlag, New York, 1993), pp. 277-292], and (2) a molecular (statistical network) theory commonly used for the rheological modeling of polymeric materials [Zhu et al., J. Biomech. 24, 1007-1018 (1991)]. Analytical expressions of elastic and viscous shear moduli, dynamic viscosity, and damping ratio based on the two theories with specific model parameters were applied to curve-fit the empirical data. Results showed that the theoretical predictions matched the empirical data reasonably well, allowing for parametric descriptions of the data and their extrapolations to frequencies of phonation.

Comparison between electroglottography and electromagnetic glottography.

Newly developed glottographic sensors, utilizing high-frequency propagating electromagnetic waves, were compared to a well-established electroglottographic device. The comparison was made on four male subjects under different phonation conditions, including three levels of vocal fold adduction (normal, breathy, and pressed), three different registers (falsetto, chest, and fry), and two different pitches. Agreement between the sensors was always found for the glottal closure event, but for the general wave shape the agreement was better for falsetto and breathy voice than for pressed voice and vocal fry. Differences are attributed to the field patterns of the devices. Whereas the electroglottographic device can operate only in a conduction mode, the electromagnetic device can operate in either the forward scattering (diffraction) mode or in the backward scattering (reflection) mode. Results of our tests favor the diffraction mode because a more favorable angle imposed on receiving the scattered (reflected) signal did not improve the signal strength. Several observations are made on the uses of the electromagnetic sensors for operation without skin contact and possibly in an array configuration for improved spatial resolution within the glottis.

Irregular vocal-fold vibration--high-speed observation and modeling.

Direct observations of nonstationary asymmetric vocal-fold oscillations are reported. Complex time series of the left and the right vocal-fold vibrations are extracted from digital high-speed image sequences separately. The dynamics of the corresponding high-speed glottograms reveals transitions between low-dimensional attractors such as subharmonic and quasiperiodic oscillations. The spectral components of either oscillation are given by positive linear combinations of two fundamental frequencies. Their ratio is determined from the high-speed sequences and is used as a parameter of laryngeal asymmetry in model calculations. The parameters of a simplified asymmetric two-mass model of the larynx are preset by using experimental data. Its bifurcation structure is explored in order to fit simulations to the observed time series. Appropriate parameter settings allow the reproduction of time series and differentiated amplitude contours with quantitative agreement. In particular, several phase-locked episodes ranging from 4:5 to 2:3 rhythms are generated realistically with the model.